Fig. 8.

Multiple calcium chelator and channel blockers affected the gravity response in acs7-1. The WT (WS) and the acs7-1 mutant were grown in half-strength Murashige and Skoog (1/2MS) medium for 3 d and transferred to 1/2MS medium which contained (A) control (normal conditions), (B) 10mM LiCl, (C) 0.25mM EGTA and other calcium channel blockers, and grown for 1 d. The gravity vector is downwards as the arrowhead indicates. The root curvature (degree) and ratio of gravity response (%) were measured in both the WT and the acs7-1 mutant after 24h (D and E). The average and SE are presented (n=60) for multiple calcium chelators or channel blockers in three independent experiments in (C), and the ratio of the gravity response is presented (n=60) for multiple calcium chelators or channel blockers in (D). RR, ruthenium red. Significant differences between normal conditions and treatment groups are indicated by * at P < 0.05 and by ** at P < 0.01 by Student’s t-test.