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. 2013 Aug 20;64(14):4375–4387. doi: 10.1093/jxb/ert243

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Effect of OsMTP8.1 expression on tolerance to Mn, Zn, and Co and accumulation of Mn in Saccharomyces cerevisiae. (A) Yeast complementation assay. The yeast mutants Δpmr1 or Δzrc1cot1 carrying the pYES2 empty vector or pYES2-OsMTP8.1 were used. A 5 µl aliquot (OD₆₀₀=2.0) of serial dilutions (10-fold) was spotted onto SC-U/Gal medium with or without (control) supplementation with 5mM MnCl₂, 0.25mM ZnSO₄, or 0.4mM CoCl₂. Plates were incubated for 48h at 30 °C in the dark. (B) Mn accumulation by the S. cerevisiae strain BY4741. BY4741 was transformed by the pYES2 empty vector or pYES2-OsMTP8.1 was cultured in liquid SC-U/Gal medium supplemented with 5mM MnCl₂ at an initial OD₆₀₀=0.1 for 24h. Data represent means ±SD (n=3). Significant differences between the empty vector and OsMTP8.1 calculated using Student’s t-test are indicated by ** (P < 0.01).