Table 2.
Metabolites accumulating in the flesh of developing tomato fruits expressing AroG209-9
| Metabolites | Flesh | ||
|---|---|---|---|
| Mature green | Breaker | Ripe red | |
| Amino acids | |||
| Phe (G) | 0.88±0.10 | 2.41±0.26a | 22.08±3.22a |
| Tyr (G) | 0.87±0.12 | 2.69±0.38 | 15.27±3.19a |
| Asn (G) | 0.60±0.06 | 0.94±0.12 | 0.49±0.06a |
| Ile (G) | 1.03±0.14 | 1.08±0.12 | 1.97±0.11a |
| Organic acids | |||
| Prephenic acid (G) | 3.4±0.4a | 276.3±90.9a | 235.9±31.3a |
| Shikimic acid (G) | 1.13±0.13 | 2.53±0.55 | 62.93±11.69a |
| Sugars | |||
| Cellobiose (G) | 0.96±0.07 | 1.15±0.05 | 22.0±3.1a |
| Maltose (G) | 1.23±0.11 | 0.95±0.05 | 1.54±0.08a |
| Raffinose (G) | 0.38±0.09 | 2.79±0.56 | 92.3±15.4a |
| Trehalose (G) | 1.76±0.33 | 0.91±0.16 | 5.31±0.88a |
| Polyamines | |||
| Putrescine (G) | 0.83±0.06 | 0.79±0.11 | 0.16±0.01a |
| Phenylpropanoids | |||
| Coumaric acid (G) | 0.35±0.05 | 2.62±0.68 | 613.6±54.2a |
| Coumaric acid hexoside or derivative (L) | 1.07±0.18 | 1.84±0.29 | 114.5±15.27a |
| Kaempferol-glucose-rhamnose (L) | 1.09±0.10 | 0.92±0.04 | 108.6±27.70a |
| Naringenin chalcone (L) | 0.51±0.14 | 10.08±2.91a | 1.78±0.42 |
| Quercetin (L) | 0.23±0.03a | 0.72±0.14 | 1.13±0.19 |
| Carotenoids | |||
| Phytoene (H) | ND | ND | >0.001a |
| Phytofluene (H) | ND | ND | >0.001a |
| Lycopene-like (H) | ND | ND | 0.55±0.10a |
Tomato fruits from T3 generation were used. Metabolite levels are presented as fold-change (AroG 209-9/WT) in each developing stage: mean ± SE. a P < 0.05 (false discovery rate) as analysed by two-way ANOVA and a Student t-test. G, GC-MS (n = 6); H, HPLC-PDA (n = 4); L, UPLC/qTOF-MS (n = 5); ND, not detected.