Fig. 4.

Relative quantification of GA oxidase transcripts in different V. vinifera organs as determined by qRT–PCR. Relative expression of GA oxidase genes whose full-length coding sequence could be cloned grouped according to their functional class (experimentally confirmed or predicted): GA20ox (A), GA3ox (B) C ₁₉ -GA2ox (C), and (predicted) C ₂₀ -GA2ox (D). Vertical bars represent the normalized relative quantity (NRQ) of GA oxidase transcripts in three biological replicates (error bars indicate the SD, n=3). Normalization was performed against the relative quantities of ACTIN and SAND. The analysed organs were: young leaf (YL); mature fully expanded leaf (ML); green bud (Bu); green internode (In); root (Ro); and tendril (Te). The floral organs were obtained from inflorescences at anthesis (50% of flowers retaining their calyptra) and were: whole unopened flower (Fl); rachis (Ra); detached calyptra (Cal); stamen and pollen of open flowers (S/P); and carpel (Car) of open flowers. The berry organs were: seed (Se) from berries at the green-hard stage; whole berry at the pea-size stage (BP; stage EL29 according to Coombe, 1995); berry (deprived of seeds) at the green-hard stage (BGH, stage EL33), and, finally, berry (no seeds) at post-véraison (BPV, stage EL36–37). NRQ of the unopened flower is normalized to 1.