Fig. 5.

Effect of a continuous ethylene treatment (A) and heat treatment (37 ºC) followed by ethylene application (B) on the expression of the CCD4b1 gene in peel of Navel oranges or Clementine mandarin. (A) Mature-green fruits (harvested in October) of Navel sweet orange and Clementine mandarin were exposed to air or to ethylene (10 µl l^–1) at 20 ºC for up to 3 d. (B) Coloured Navel sweet oranges (harvested in November) were incubated at 20 ºC (control) or heat treated at 37 ºC and 90% relative humidity for 3 d. After heating at 37 ºC, fruits were exposed to an ethylene treatment (10 µl l^–1) at 20 ºC for an additional 4 d. The images are representative of the external colour of the fruits at the beginning and the end of each treatment. Expression values are relative to transcript levels obtained in the peel of Navel sweet orange fruits at the onset of the experiments, which was arbitrarily set to 1. N.d. indicates not detected. Data of transcripts levels are means ±SD of three replicates. (This figure is available in colour at JXB online.)