Fig. 7.

HPLC analysis of the in vitro enzymatic activity of Citrus CCD4b1. Assays were incubated for 1h, except for lycopene which was incubated for 6h. The crude lysate of thioredoxin-CCD4b1-expressing E.coli cells (CCD4b1) converted (A) β-carotene into β-apo-8′-carotenal (P1), (B) β-cryptoxanthin into P1 and β-citraurin (P2), (C) zeaxanthin into P2, (D) lutein into 3-OH-ε-apo-8′-carotenal (α-citraurin) (P3), and (E) lycopene into apo-8′-lycopenal (P4) and apo-10′-lycopenal (P5). UV-Vis spectra of obtained products are depicted in the insets. No conversion was observed with the corresponding controls corresponding to crude lysates of thioredoxin-overexpressing cells (Con).