Fig. 2.

OsDEP1 is composed of two domains; the C-terminal half is Cys rich and is sufficient to confer Cd tolerance to yeast cells. (A) Schematic representation of OsDEP1 and its two truncated derivatives. OsDEP1 consists of 426 aa, of which 120 are Cys residues, with 88 of these being localized to the OsDEP1(170–426) C-terminal half. (B) Cd tolerance of yeast cells expressing intact OsDEP1 or the truncated OsDEP1(1–169) and OsDEP1(170–426) derivatives. Experiments were performed as described in Fig. 1. (C) Growth curves of yeast strains in SD-Ura liquid medium supplemented with 40 μM CdCl₂. The growth of the yeast cells was monitored at OD₆₀₀. The data are means ±standard deviation (SD) from three independent experiments.