Fig. 4.

Generation of transgenic Arabidopsis lines expressing intact OsDEP1 and its truncated OsDEP1(1–169) and OsDEP1(170–426) derivatives, and their responses to CdCl₂-induced stress. (A) RT-PCR analysis of OsDEP1 and OsDEP1(170–426) in each of two independent transgenic Arabidopsis lines. EV, control transgenic line; lines #23 and #28, transgenic lines carrying the intact OsDEP1 gene; lines Crich6 and Crich9, transgenic lines carrying the OsDEP1(170–426) Cys-rich domain. The Arabidopsis tubulin gene (GenBank accession no. NM_001203444, AtTUB) was used as a loading control. (B–E) Transgenic seeds were sown onto MGRL/1% gellan gum medium with or without 75 μM CdCl₂ for 2 weeks, at which time pictures and root length measurements were taken. (B, C) Growth responses (B) and root lengths (C) of control transgenic (EV) and two independent OsDEP1 transgenic Arabidopsis lines, #23 and #28, in response to control 0 μM and 75 μM CdCl₂. (D, E) Growth responses (D) and root lengths (E) of control transgenic (EV) and two independent transgenic Arabidopsis lines, Crich6 and Crich9, expressing the Cys-rich OsDEP1(170–426) region, to control (0 μM) and 75 μM CdCl₂. Asterisks in (C) and (E) indicate that the difference from the control (EV) is statistically significant: ^*** P <0.001. (F) RT-PCR analysis of OsDEP1(1–169) in each of two independent transgenic Arabidopsis lines. EV, control transgenic line; lines ΔCrich1 and ΔCrich2, transgenic lines carrying the OsDEP1(1–169) domain. (G, H) Growth responses (G) and root lengths (H) of control transgenic (EV) and two independent transgenic Arabidopsis lines, ΔCrich1 and ΔCrich2, expressing the OsDEP1(1–169) region, to control (0 μM) and 75 μM CdCl₂. (This figure is available in colour at JXB online.)