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. 2013 Sep 10;64(14):4541–4557. doi: 10.1093/jxb/ert269

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Cloning and identification of CsKF1, CsKF2, and CsKF3 genes. (A) The full-length CDS of CsKF1, CsKF2, and CsKF3 were obtained by RT-PCR using specific primers designed according to the cucumber genome database. (B) Schematic diagram for the domain organization of CsKF1, CsKF2, and CsKF3 analysed in SMART (http://smart.embl-heidelberg.de/), indicating that these kinesins have putative kinesin motor domains in the central region. The truncated peptides CsKF1-N, CsKF2-C, and CsKF3-C for preparation of polyclonal antibodies are also indicated in the diagram. (C) Prediction of coiled-coils in CsKF1, CsKF2 and CsKF3 by COILS (http://www.ch.embnet.org/software/COILS_form.html). The x-axis represents the position of amino acid residues, and the y-axis represents the probability of the formation of coiled-coils. A probability value >0.5 indicates that the corresponding region most probably forms a coiled-coil.