Fig. 7.

In vitro ATPase activities and microtubule (MT) binding abilities of CsKF1 and CsKF2 motors. (A) Prokaryotic expression and affinity purification of His-tagged CsKF1 and CsKF2 motor domain fusion proteins. (B) MT concentration dependence of CsKF1-motor and CsKF2-motor ATPase activities. (C) MT binding assay. The resulting supernatant (S) and pellet (P) were analysed by SDS–PAGE and visualized by Coomassie blue staining. For each assay, 7 μM motor proteins were used for MT binding in the absence or presence of 5mM MgATP or AMP-PNP.