Fig. 8.

Preparation of CsKF1, CsKF2, and CsKF3 antibodies. Prokaryotic expression and affinity purification of His-tagged CsKF1-N, CsKF2-C, and CsKF3-C fusion proteins used as antigen proteins (A). The polyclonal antibodies could specifically identify the corresponding antigen peptides KF1-N (B), KF2-C (C), and KF3-C (D). Western blot analysis of CsKF1, CsKF2, and CsKF3 in proteins of root, leaf, stem, shoot, male flower, female flower, and fruit. An ~80kDa protein from cucumber root, leaf, shoot, and fruit reacted with the anti-KF1 antibody (E); an ~125kDa protein from cucumber leaf, shoot, and fruit reacted with the anti-KF2 antibody (F); and an ~110kDa protein from cucumber root, stem, flower, and fruit was recognized by the anti-KF3 antibody (G). An anti-α-tubulin antibody was used in a positive control experiment, and the 50kDa protein band was revealed in all samples (H).