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. 2013 Sep 4;64(14):4575–4587. doi: 10.1093/jxb/ert271

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Colocalization of AtTPX2 and γ-tubulin in Arabidopsis AtTPX2-GFP-overproducing cells. (A, B) γ-Tubulin localized together with AtTPX-GFP. (A) 3D analysis of cells overexpressing AtTPX2-GFP stained with anti-γ-tubulin antibody (red), DAPI (blue) obtained by laser scanning microscopy and 3D-reconstructed (Imaris, Bitplane). Still images from 3D reconstruction animation show γ-tubulin localized with AtTPX2-GFP-decorated fibres in vicinity of nuclear envelope (A, arrows), with intranuclear microtubules, and in patchy pattern on microtubular bundles extending from perinuclear area (arrowhead). (B) γ-Tubulin colocalized with AtTPX2 on microtubular fibres extending from perinuclear area to the cytoplasm and membranes (arrow) and on the nuclear envelope (arrowhead). Colocalization was analysed by ImageJ software with JACoP plugin (Bolte and Cordeliéres, 2006). The similar labelling pattern was observed in 93% of analysed overexpressing cells (n = 128). Bars, 10 µm.