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. 2013 Sep 16;64(14):4559–4573. doi: 10.1093/jxb/ert274

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Determination of the critical water content of mature Castanospermum australe cotyledons. Castanospermum australe seed after shedding with (A) and without a seed coat (B). (C) Embryonic axis and cotyledons of mature seeds. The scale bar represents 1cm. (D) Tetrazolium staining (red indicating living tissues) of cubes (50mm³) taken from the core of mature cotyledons that were first dried at 44% RH for the indicated time. (E) The relationship between water content during drying and pixel intensity of the tetrazolium (TZ) staining. Data for four independent experiments (represented by different symbols) are shown.