Table 2.
LEA transcripts identified in the Castanospermum australe seed transcriptome
| Family (PFAM) | Protein name | Blast database | M. truncatula ID | C. australe contigsa | E-value | Alignment length | Percentage identity | Percentage similarity | Spot on 2D gel |
|---|---|---|---|---|---|---|---|---|---|
| Dehydrin | DHN3 | MtGI11 | TC175037 | Ca_11990 | 2.E-09 | 198 | 47.2 | 52.0 | 3, 4 |
| DHN-cognate | Mt3.5 | Medtr3g117290 | Ca_9276 | 2.E-10 | 120 | 53.3 | 65.0 | 5 | |
| BudCar5 | Mt3.5 | Medtr7g086340 | Ca_31427 | 5.E-06 | 119 | 62.2 | 77.7 | 1 | |
| LEA_5 | EM6 | Mt3.5 | Medtr4g016960 | Ca_14307 | 4.E-45 | 98 | 81.6 | 90.8 | 37 |
| EM1 | MtGI11 | AJ498523 | Ca_2036 | 9.E-40 | 100 | 78.2 | 85 | 51 | |
| LEA_4 | SBP65 | Mt3.5 | Medtr4g079690 | Ca_7340 | 5.E-17 | 90 | 58.9 | 72.2 | 75 |
| PM10 | MtGI11 | TC174929 | Ca_3035 | 4.E-43 | 274 | 59.9 | 74.8 | ND | |
| PM18 | MtGI11 | TC183861 | Ca_330 | 2.E-41 | 335 | 56.0 | 67.2 | ND | |
| MP2 | Mt3.5 | Medtr1g061730 | Ca_8462 | 3.E-31 | 226 | 57.5 | 67.1 | 9 | |
| LEAm | Mt3.5 | Medtr2g014040 | Ca_7604 | 3.E-45 | 268 | 48.6 | 62.4 | 74 | |
| CAPLEA.I | MtGI11 | TC175990 | Ca_8841 | 1.E-31 | 141 | 66.0 | 85.8 | 18,19 | |
| LEA_1 | PM1 | Mt3.5 | Medtr7g093170 | Ca_8304 | 3.E-16 | 91 | 64.8 | 75.8 | ND |
| D113.II | Mt3.5 | Medtr7g093160 | Ca_8304 | 5.E-17 | 91 | 65.2 | 76.1 | ND | |
| SMP | PM25 | MtGI11 | TC174777 | Ca_6007 | 2.E-83 | 238 | 75.6 | 84.4 | 92 |
| D-34.I | MtGI11 | Medtr1g072090 | ND | ND | |||||
| D-34.II | MtGI11 | TC183570 | Ca_25629 | 6.E-42 | 128 | 86.7 | 93 | ND | |
| D-34.III | Mt3.5 | Medtr2g076230 | Ca_23377 | 3.E-06 | 24 | 76.0 | 87.5 | ND |
ND, homologue not detected.
Contigs were translated to calculate the percentage identity and similarity.
a Castanospermum australe contigs that were homologues to the 17 LEA gene products detected in M. truncatula seeds were identified based on the MtGI11 and Mt3.5 Medicago databases (E-value <e-06).