Table 3.
Evidence for ABI3-dependent regulation of LEA homologues for which protein abundance is reduced or absent in desiccation-sensitive tissues (C. australe and Mtabi3) (group A) or unaffected or increased (group B)
| Protein name | LEA group | Medicago truncatula | Cis-elementsb | Arabidopsis thaliana | |||||
|---|---|---|---|---|---|---|---|---|---|
| Sequence ID | Nimblegen probe | 35S::ABI3/ controla | P-value | AGI | Transcript level in abi3 seeds versus WTc | ABI3 targetsd | |||
| EM6 | A | Medtr4g016960 | Medtr_v1_022627 | 3.87 | 1.92E-06 | 2 RY, 2 ABRE | AT2G40170 | Down | – |
| EM1 | A | AJ498523 | Medtr_v1_072582 | 2.04 | 5.53E-02 | ND | AT3G51810 | Down | T, P |
| SBP65 | A | Medtr4g079690 | Medtr_v1_083614 | 4.18 | 3.07E-03 | 2 RY, 1 ABRE | AT2G42560 | Down | T, P |
| PM10 | A | Medtr8g134020 | Not present on slide | ND | AT5G44310 | NA | T, P | ||
| LEAm | A | Medtr2g014040 | Medtr_v1_009629 | 2.98 | 2.34E-03 | 1 RY, 2 ABRE | AT5G44310 | NA | T, P |
| MP2 | A | Medtr1g061730 | Medtr_v1_005821 | 3.87 | 1.51E-05 | 0 RY, 2 ABRE | AT2G36640 | Down | T, P |
| PM18 | A | TC183861 | Medtr_v1_076240 | –0.02 | 8.65E-01 | ND | AT2G36640 | Down | T, P |
| PM1 | A | Medtr7g093170 | Medtr_v1_045826 | 4.59 | 7,89E-07 | 0 RY, 1 ABRE | AT5G06760 | NA | C |
| D113.II | A | Medtr7g093160 | Medtr_v1_045826 | 4.59 | 7.89E-07 | 2 RY, 1 ABRE | AT5G06760 | NA | C |
| PM25 | A | TC174777 | Medtr_v1_082683 | 3.29 | 1.32E-02 | ND | AT3G22490 | Down | T, P, A |
| D-34.I | A | Medtr1g072090 | Medtr_v1_006041 | 2.21 | 4.07E-02 | 5 RY, 0 ABRE | AT3G22490 | Down | T, P, A |
| D-34.III | A | Medtr2g076230 | Medtr_v1_012326 | 2.32 | 4.43E-02 | 0 RY, 0 ABRE | AT3G22490 | Down | T, P, A |
| DHN3 | B | TC175037 | Medtr_v1_066754 | 3.32 | 1.10E-05 | ND | ND | ND | ND |
| DHN-cognate | B | Medtr3g117290 | Medtr_v1_020587 | –1.47 | 4.91E-04 | 0 RY, 1 ABRE | AT1G76180 | Up | – |
| BudCar5 | B | Medtr7g086340 | Medtr_v1_045277 | 1.57 | 3.70E-02 | 0 RY, 0 ABRE | ND | ND | ND |
| CAPLEA-1 | B | TC175990 | Medtr_v1_085905 | 1.06 | 6.96E-02 | ND | AT1G52690 | Up | – |
a Log ratio of transcript levels (and corresponding P-values) in hairy roots overexpressing MtABI3 compared with control (empty plasmid), determined by trancriptome analysis using Nimblegen slides (GeOmnibus GSE44291).
b The number of RY (CATGCA) and ABRE (ACGTG(G/T)C) cis-regulatory motifs known to bind ABI3 was revealed by analysing the 2kb promoter sequence of the M. truncatula genes.
c Relative level of transcripts in mature abi3 seeds of Arabidopsis compared with the wild type. Data are extracted from Bies-Etheve et al. (2008).
d Identification of ABI3-responsive gene products in 35S::ABI3-GR seedlings by array-based transcriptome analysis (T) or qRT-PCR (P) and confirmation as direct targets by transient promoter activation assay (A) or ChIP-chip analysis (C). Data are extracted from Mönke et al. (2012).
ND, not detected; NA, not analysed; WT, wild type.