Table 2. Rnq1-GFP fluorescence patterns show diversity among [RNQ+] variants.

[RNQ+] Variant	Rnq1-GFP Characterization
[rnq-]	Diffuse fluorescence
s.d. low	One large focus (s.d.)
m.d. high	Multiple large foci (m.d.) and numerous petite foci (p.f.)
strong	Majority p.f.
medium	Majority s.d.
weak	Diversity: p.f., s.d., and m.d. present
very weak	Majority s.d., also m.d. present
A5	Majority s.d., also m.d. present
B1 P/W	Roughly 50% p.f., 50% s.d.
B1 W	Diversity: p.f., s.d., and m.d. present
C3	Majority s.d., also m.d. present
D3	Roughly 50% p.f., 50% s.d.
E1	Majority p.f., low percentage of s.d.
G4	Majority p.f., low percentage of s.d.
G6	Majority p.f., low percentage of s.d.

[RNQ+] variants expressing Rnq1(153-405)-GFP were characterized by the relative number of GFP aggregate-containing cells with single-dot (s.d.), multiple-dot (m.d.), or petite-foci (p.f.) fluorescence patterns. For each [RNQ+] variant, Z-stacks of at least 150 cells were analyzed.