Figure 4. Immunofluorescent labeling of endometrial tissue for γδT-cells.

(a-f) Representative images of immunofluorescent γδT cell receptor (WC1) labeling (green) in cells (nuclei labeled with DAPI [blue]) of the endometrial deep stroma (DS) from cyclic (C) Day 5 and pregnant (P) Day 7 and 16 -heifers (original magnification ×40). (g-i) Line graphs detailing the total number of γδT-cells in cyclic (red line) and pregnant (blue line) endometrial tissue based on the sum of immunofluorescent labeled cells per 2.25 mm² field of view, in all areas (total γδT-cells⁺) and specifically in the SS and DS. Values plotted are least squared means and standard errors across 5 replicate animals per timepoint and per status.