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. 2013 Oct 25;8(10):e77647. doi: 10.1371/journal.pone.0077647

Figure 1. Production of transgenic A. burtoni.

Figure 1

A) Tol2-flanked transgenic construct DNA (pT2KXIGInline graphicn) is co-injected with mRNA encoding transposase into fertilized single cell embryos. B) Images of embryos 6 days post-fertilization. i) Brightfield image of EF1α:GFP-injected embryo; ii) Robust GFP expression in embryo shown in i; iii) Uninjected embryo. Arrows mark the heads of embryos. Note that yolk exhibits autofluorescence.