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. 2013 Oct 25;8(10):e77647. doi: 10.1371/journal.pone.0077647

Table 1. Oligonucleotides used.

Primer name Sequence
Nkcrg2-f AGT GGT GGA AGG GAA CAA TG
Nkcrg2-r TTA GCC TGA GCA GCC ACT TC
Tom1-f AAT ATA CCT CTG TGT CTA CCA G
Tom1-r AGC ATA ACT AAA TAA GCT GTC AG
EF1a-f CCT ACA GCT CCT GGG CAA CG
GFP-r AGC TTG CCG TAG GTG GCA TC
Splink1 TAA CCG TTG CTA GGA GAG ACC GTG GCT GAA TGA GAC TGG TGT CGA CAC TAG TGG CAT G
Splink2 CCA CTA GTG TCG ACA CCA GTC TCT AAT TTT TTT TTT CAA AAA AA
Splink3 AAC CGT TGC TAG GAG AGA CC
Tol2-r GCG TGT ACT GGC ATT AGA TTG
Splink5 AAT TAA ACT GGG CAT CAG CG
Splink6 GCT GAA TGA GAC TGG TGT CG
Splink7 TAA ATA CAA ACA GTT CTA AAG CAG
Splink8 ATT GGT TTG GTA ATA GCA AGG G

Primers used to detect and confirm transgene insertion are detailed.