Table 1. Oligonucleotides used.
Primer name | Sequence |
Nkcrg2-f | AGT GGT GGA AGG GAA CAA TG |
Nkcrg2-r | TTA GCC TGA GCA GCC ACT TC |
Tom1-f | AAT ATA CCT CTG TGT CTA CCA G |
Tom1-r | AGC ATA ACT AAA TAA GCT GTC AG |
EF1a-f | CCT ACA GCT CCT GGG CAA CG |
GFP-r | AGC TTG CCG TAG GTG GCA TC |
Splink1 | TAA CCG TTG CTA GGA GAG ACC GTG GCT GAA TGA GAC TGG TGT CGA CAC TAG TGG CAT G |
Splink2 | CCA CTA GTG TCG ACA CCA GTC TCT AAT TTT TTT TTT CAA AAA AA |
Splink3 | AAC CGT TGC TAG GAG AGA CC |
Tol2-r | GCG TGT ACT GGC ATT AGA TTG |
Splink5 | AAT TAA ACT GGG CAT CAG CG |
Splink6 | GCT GAA TGA GAC TGG TGT CG |
Splink7 | TAA ATA CAA ACA GTT CTA AAG CAG |
Splink8 | ATT GGT TTG GTA ATA GCA AGG G |
Primers used to detect and confirm transgene insertion are detailed.