Figure 5.
(a) and (b) Procedures to harvest cultured spheroids from the microfluidic device using a biopsy punch and a pipette. (c) Confocal microscopic image of Oct-4 GFP EBs formed and cultured in a microfluidic device. (d) Confocal microscopic image of Oct-4 GFP EBs harvested from a microfluidic device at day 2. Scale bar is 150 μm. (e) HepG2 (at day 3) spheroids harvested out from the device and quantitative size distribution before and after the harvesting.