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. 2013 Oct 25;8(10):e77468. doi: 10.1371/journal.pone.0077468

Figure 1. PVT1-derived miRNAs.

Figure 1

A. Location of the validated PVT1-derived miRNAs. The grey bars represent the nine PVT1 exons. Panels B, C and D, relative quantification of PVT1-derived miRNAs in human renal proximal tubule epithelial cells (RPTEC), podocytes, and mesangial cells (MC) by TaqMan qPCR, respectively. RNU6B and RNU44 were used as endogenous controls. E. Relative quantification of miRNAs in MC grown for 48 h in MsBM medium supplemented with 5% FBS, containing either normal glucose (NG: 5.6 mM), NG +3-O-methyl-D-glucose (3-O-MG) to control for osmotic effects (OC: 5.6 mM glucose +24.4 mM 3-O-MG), or high glucose (HG: 30 mM). Results represent average of three independent experiments. Data are means ± S.D. A.U.: arbitrary units. The significance is indicated only for samples that are significant different from all the others. * P<.05; ** P<0.01; *** P<0.001.