Table 1. Analysis of ECM accumulation-related miR-1207-5p targets using TargetScan and miRanda.
| TargetScan1 | miRanda2 | ||||||||
| Conserved sites3 | Poorly conserved sites | ||||||||
| Gene symbol | Gene name | Context score4 | n | Type4 | Target position (3′UTR region) | n | Type5 | Target position (3′UTR region) | mirSVR score |
| G6PD | Glucose-6-phosphate dehydrogenase | −0.71 | 1 | 8mer | 584–591 | 1 | 7mer-m8 | 212–218 | −0.91 |
| PMEPA1 | Prostate transmembrane protein, androgen induced 1 | −0.34 | 0 | 2 | 7mer-m8 | 2976–2982; 3358–3364 | −0.12 | ||
| PDPK1 | 3-phosphoinositide dependent protein kinase-1 | −0.42 | 0 | 6 | 8mer, 7mer-m8, 7mer-1A | 85–91, 369–375, 1416–1422, 1687–1693, 3078–3085, 4052–4058 | −0.13 | ||
| SMAD7 | SMAD family member 7 | −0.55 | 0 | 4 | 8mer, 7mer-m8, 7mer-1A | 580–586, 694–700, 703–710, 833–839 | −0.42 | ||
1
TargetScan website: http://www.targetscan.org.
2
miRanda website: http://www.microrna.org.
3
Conserved across most mammals.
4
Predicted efficacy of targeting [20].
5
Type of site: TargetScan predicts biological targets of miRNAs by searching for the presence of conserved 8mer and 7mer sites that match the seed region of each miRNA. 7mer-1A sites: exact match to positions 2–7 of the mature miRNA (the seed) followed by an “A” (adenine). 7mer-m8 sites: exact match to positions 2–8 of the mature miRNA (the seed + position 8). 8mer sites: exact match to positions 2–8 of the mature miRNA followed by an “A” (adenine). 7mer-m8 sites are defined [19].