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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Genes Immun. 2013 Jul 18;14(7):427–433. doi: 10.1038/gene.2013.36

Figure 3.

Figure 3

EMSA analysis of the putative MZF-1 binding sites of KIR2DL1 and KIR3DL1. (a) A schematic of the core promoter fragment is shown, with the shaded rectangles indicating potential transcription factor binding sites. The expanded sequence underneath shows the oligonucleotide probes from the predicted KIR2DL1 and KIR3DL1 MZF-1 binding sites compared with the known MZF-1 binding site in the human CD34 gene. The consensus nucleotides for MZF-1 binding are shown below (Consen). Brackets indicate the sequences bound by the first and second DNA-binding domains of MZF-1. Nucleotide substitutions present in mutated KIR2DL1 (2DL1m) and CD34 (CD34m) probes are shown below the respective sequences. (b) EMSA analysis of HEK293 (left panel) and YT (right panel) nuclear extracts using the oligonucleotide probes shown in (a). Unlabeled oligonucleotides used for cold competition are indicated (Comp), as are the labeled probes (Probe). The position of a HEK293 cell-specific MZF-1 complex (MZF-1 -) is indicated next to the left panel, and a non-specific complex formed in YT cells is indicated next to the right panel (nonspecific).