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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Genes Immun. 2013 Jul 18;14(7):427–433. doi: 10.1038/gene.2013.36

Figure 4.

Figure 4

Enforced expression of MZF-1 decreases KIR expression in developing NK cells. (a) FACS analysis of day 21 in vitro differentiated cord blood NK cells transduced with either a control GFP expressing lentiviral vector or a lentiviral vector expressing GFP-IRES-MZF-1. Cells gated on the GFP−ve subset are shown on the left, and cells gated on the GFP+ve subset are shown on the right. Cells were stained with a cocktail of APC-conjugated NCAM16.2 (CD56) and PE-conjugated DX9 (KIR3DL1), EB6 (KIR2DL1/S1) and GL183 (KIR2DL2/S2/L3). (b) The average fold increase in MZF-1 mRNA expression in cells over-expressing MZF-1 relative to control GFP cells calculated from 3 independent donors. Differences in MZF-1 mRNA expression were statistically significant (p=0.0189) as determined by two-tailed Student’s t test. (c) The average fold reduction of KIR expression in cells over-expressing MZF-1 relative to control GFP cells calculated from 3 independent donors. Differences in KIR expression were statistically significant (p=0.0274) as determined by two-tailed Student’s t test. (d) PCR analysis of GAPDH (Lanes 3 & 4), KIR2DL1 distal antisense transcript (Lanes 6 & 7) and KIR3DL1 distal antisense transcript (Lanes 9 & 10) in sorted GFP and MZF-1 over-expressing day 21 in vitro-derived NK cells. Data is representative of 3 donors tested.