Table 1.
gene | name | sequencea |
---|---|---|
liaI | liaI-for-HinDIII | ATGGAAGCTTCATATGATCTCAAATATTTCGATCC |
liaI-rev-BamHI | ATATGGATCCCAATTAATAAGAATCCGCCTATTG | |
sigW | sigW-for-HinDIII | ATATAAGCTTCCATTATTGGGCTATAGCCAAGCGG |
sigW-rev-BamHI | ATATGGATCCCCTCATGCACATTGCCAAGCATACG | |
recA | recA-forb | GGCGCTAAATACGGCTGCCATTTAATC |
recA-rev-BamHI | ATATGGATCCGCTTTCAGGACCGTATACTTC | |
clpP | clpP-for-HinDIII | ATATAAGCTTCCTGTTGTTCTATTGGACGCGCT |
clpP-rev-BamHI | ATATGGATCCGGATCGTATCATCATGCTTGGATC | |
katA | katA-for-HinDIII | ACGTAAGCTTCCATCTCTGTTCTTGTAGAGAT |
katA-rev-BamHI | ATATGGATCCCGTGAACAACACGTTCAGGAAC | |
sigB | sigB-for-HinDIII | ATATAAGCTTCGTTTAGAGGTTATTGTGGCGGAT |
sigB-rev-BamHI | ACGTGGATCCGCATGTCAACCAGATTTGTATACA |
aAdded restriction sites are underlined. Additional nucleotides added for cleavage efficiency are italicized.
brecA sequence carries a HinDIII restriction site directly after the primer.