Figure 1.
In situ detection of enzymatic activities using the preferential substrate peptides and immunostaining analysis using antibodies against transglutaminase skin-type (TG1) and tissue-type (TG2). (A) Sagittal plane sections from the wild-type and TG1-null mice at E19.5 stage were reacted with 1 μM fluorescent isothiocyanate (FITC)-pepK5 in the presence of 5 mM CaCl2. As negative controls, samples were treated with 1 μM of FITC-pepK5QN or in the presence of 5 mM EDTA. Immunofluorescent staining using a polyclonal antibody against TG1 was also performed. (B) Neonate whole-mouse body sagittal plane sections from wild-type and TG2-null mice were reacted with 1 μM FITC-pepT26. As described in the legend to (A), negative controls (1 μM FITC-pepT26QN or EDTA) and immunostaining with an antibody against TG2 were performed. Bars = 0.5 cm.