Figure 10.

Co-immunoprecipitation of wild-type cryptochrome, nitric oxide synthase–interacting protein (NOSIP), and GNB-Trans by protein A/G agarose beads, coated with anti–GNB-Trans antibodies. Extracts from primmorphs were prepared from either 6-day-old primmorphs, kept for the last 3 days in the dark (time 0), or from those primmorphs that had been exposed for 1 or 2 hr to white light (time 1 and 2 hr). Then the samples were subjected to immunoprecipitation with anti–GNB-Trans beads. After centrifugation, the bound proteins were eluted from the beads and the supernatants were processed by western blotting using the polyclonal antibodies to GNB (upper panel), Cry (middle panel), and NOSIP (lower panel). Further details are given in the Materials and Methods.