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Delayed formation of primmorphs in the presence of the nitric oxide synthase (NOS) inhibitor L-NMMA (10 µM). One day after starting the reaggregation of dissociated cells (performed under 30 lux of light), the assays, performed in parallel, were started in rectangular well plates; one series remained in the absence of the inhibitor, while the second one was supplemented with 10 µM L-NMMA, as indicated. Photomicrographs were taken every 20 min. Scale bar = 1 mm.
