Figure 7.

Detection of wild-type nitric oxide synthase–interacting protein (NOSIP) and TBLR protein in extracts, prepared from primmorphs. (A) Proteins were extracted from 6-day-old primmorphs and were size-separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); the gels were stained with Coomassie brilliant blue (lane a). (B) The wild-type NOSIP protein was detected by western blotting (WB) using PoAb-r-NOSIP-r_SUBDO antibodies. The 34-kDa polypeptide was detected (lane a); no antigen-antibody signals are seen if the membranes are incubated with adsorbed (ads) antibodies (lane b). (C) The transducin β-subunit (GNB) was identified in the extract as a 37.5-kDa polypeptide using the PoAb-r-GNB-Trans_SUBDO antiserum (lane a); the adsorbed sample showed no reaction (lane b). In A, lane a, the size marker used is shown.