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. 2013 Sep 30;110(43):17474–17479. doi: 10.1073/pnas.1312830110

Fig. 2.

Fig. 2.

Fragmentation of murine ovaries increased actin polymerization, disrupted Hippo signaling, and increased CCN growth factors and apoptosis inhibitors. (A) Ovarian fragmentation increased F-actin levels. Paired ovaries from day 10 mice were cut into three pieces or kept intact before immunoblotting analyses of F- and G-actin levels (Upper). (A, Lower) F- to G-actin ratios; n = 6–11. (B) Ovarian fragmentation decreased pYAP levels and pYAP to total YAP ratios. Paired ovaries with or without cutting were incubated for 1 h, followed by immunoblotting. (B, Left) Representative immunoblots. (B, Right) Ratios of different antigens; n = 8 pairs. (C) Ovarian fragmentation increased expression of CCN growth factors and BIRC apoptosis inhibitors. Paired ovaries with or without cutting were incubated for 1 h with subsequent grafting before analyses of transcript levels normalized by GAPDH. Intact ovaries, solid lines; pooled three pieces, dashed lines; n = 10–15. (D) Ovarian fragmentation increased CCN2 proteins. Paired ovaries with or without cutting were incubated for 3–5 h before immunoblotting. (D, Upper) Representative blots. (D, Lower) Quantitative analyses; n = 3–5. (E) Treatment with CCN2, 3, 5, and 6 increased ovarian explant weights. Explants from day 10 mice were cultured with different CCN growth factors for 4 d before weighing; n = 5–6. Mean ± SEM; *P < 0.05. IN, intact; PI, pieces.