Fig. 5.
Effects of SUMOylation and ubiquitinylation on Snf1 stability. Snf1 levels were measured in ubp8Δ cells carrying no other mutation (WT), or also carrying slx8∆ or mms21-CH, or a plasmid carrying GAL1::ULP1, as indicated before the slash (/) for each set of data; the nature of the Snf1 expressed in each mutant (Snf1-8myc or Snf1K549R or Snf1I129A, or Snf1I129A,K549R) is indicated after the slash. Cells were grown in 2% galactose at 34 °C (A) or 30 °C (B–D). Samples were processed for immunoblots at the indicated times after addition of cycloheximide (CHX) to 200 μg/mL and glucose to 2%. Pgk1 and Snf1 were quantified using ImageJ.