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Depth color-coded z-projections of deconvolved live images of yeast Zip1 filaments. Upper arrow points to a filament in ER-Decon output traversing from 6 µm depth to 8 µm depth, which cannot be resolved from the outputs of the other methods. Lower arrow points to two filaments in ER-Decon output located at depths 6.5 µm and 7.5 µm, which again cannot be resolved from the output of the other two methods. ER-Decon’s parameters: λ = 12; ε = 0.001.
