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. 2013 Oct 7;110(43):17504–17509. doi: 10.1073/pnas.1316709110

Fig. 2.

Fig. 2.

AgTreT1 silencing affects hemolymph trehalose levels. (A) AgTreT1 mRNA levels reduced in AgTreT1 dsRNA-injected mosquitoes to as low as 16% of controls during 6 d after injection. RNA of whole mosquitoes was used for reverse transcription and quantitative PCR, and data were normalized using the expression of S7 ribosomal gene as internal control. Black bars represent normalized mRNA level of mosquitoes injected with AgTreT1 dsRNA, and gray bars represent mosquitoes injected with GFP dsRNA as controls. (Inset) Western blot of whole female A. gambiae injected with GFP RNAi or AgTreT1 RNAi on day 0 (before injection) and day 2, 4, and 9 postinjection. (Upper) Blot against anti-AgTreT1 antibody. (Lower) Blot against anti-Actin antibody. (B) The relative concentration of hemolymph trehalose in AgTreT1 or GFP dsRNA-injected A. gambiae mosquitoes 8 d after injection. Trehalose concentrations were normalized to genomic DNA extracted from hemolymph cells. Data represent mean ± SD, *P ≤ 0.05 by Student's t test.