Fig. 3.

Survival of mosquitoes with silenced AgTreT1 expression under environmental stresses. Female A. gambiae mosquitoes were incubated overnight in (A) dehydrating (<20% relative humidity) or (B) high-temperature environment (39 °C). The reduction of AgTreT1 was confirmed by RT-qPCR and Western blot. Closed triangles represent percentages of living mosquitoes injected with GFP dsRNA at specified time points, and open circles represent percentages of living mosquitoes injected with AgTreT1 dsRNA. Mosquitoes with reduced AgTreT1 expression succumbed sooner than controls under desiccation (A) and heat stresses (B). *P ≤ 0.05 by the log-rank test in survival analysis. Each plot is a representative of three different experiments with similar trends. Sample sizes and P values of three repeats are summarized in Table S1.