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. 2013 Oct 7;110(43):17588–17593. doi: 10.1073/pnas.1310644110

Fig. 3.

Fig. 3.

CDC5 is required for the recruitment of Pol II to MIR promoters. (A–C) The occupancy of Pol II at various promoters detected by ChIP using anti-RBP2 antibody in cdc5-1 and Col. (D–F) The association of CDC5 with various promoters detected by ChIP using anti-YFP antibody in plants containing pCDC5::CDC5-YFP. DNA copurified with CDC5 or Pol II was analyzed with qRT-PCR. The intergenic region between At2g17470 and At2g17460 (Pol II C1) that is not occupied by Pol II was used as a negative control. ChIP with no antibodies was performed as another control. Means and standard derivations of three technical repeats are presented, and three biological replicates gave similar results. Please note that the results of Pol II C1 in RBP2 ChIP (A and B) and in CDC5 ChIP (D and E) were showed twice, respectively, for control purposes. *P < 0.05 (t test).