Evaluation of dose of PQ and G6PD levels on hemolytic response. A- huRBC SCID mice were treated with PQ p.o. daily for 7 d with indicated doses of PQ (25, 12.5, 6.25, or 3.125 mg/kg per day) or given PBS as a vehicle control and assessed for percentage huRBCs (A) and muRetics (B) at day 7 by FACS analysis. Each treatment group consisted of 4 mice. *Statistically significant differences (one-way ANOVA with Bonferroni posttest, P < 0.001). (C) G6PD levels were measured at start of engraftment and correlated with percentage of huRBCs at 7 d after treatment with PQ (25 mg/kg per day, p.o., 7 d) using Spearman rank correlation (P = 0.0005, r = 0.7373). Data pooled from three experiments with three donors. Each dot represents an individual mouse.