Fig. 2.

Decreased leukocyte infiltration and inflammation in colons of St3gal4^−/−/Il10^−/− mice. (A) Flow cytometry analysis of lamina propria leukocytes (LPLs) isolated from a distal part of the colon of 6- and 14-wk-old Il10^−/− and S3/I10^−/− mice (n = 5–7) or 6- to 8-wk-old WT controls. Data are presented as percentage of CD45⁺, CD4⁺, CD4⁺FoxP3⁺ (T_reg), CD11c⁺, Ly6C^hi, and Ly6G⁺ cells from all isolated cells. (B) Expression levels of cytokines in colons of 6- and 14-wk-old Il10^−/−, S3/I10^−/− mice (n = 6–8) and WT control mice (n = 5–6) were determined by real-time PCR and normalized to GAPDH.