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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Integr Biol (Camb). 2012 Oct;4(10):10.1039/c2ib20171b. doi: 10.1039/c2ib20171b

Fig. 2. Short-term DEP exposure has negligible effects on NSPC survival.

Fig. 2

(A) Schematic of experimental design to test effects of short-term and long-term DEP exposure on cell survival. (B) HuNSPCs exposed to DEP frequencies for 0, 10, or 60 sec exhibit no significant decrease in cell survival as determined by trypan blue exclusion. (C) The cell survival of three different sets of NSPCs (HuNSPCs SC23 or SC27 and mNSPCs E12.5) is not affected by 60 sec DEP exposure at any frequency. (D) Left panel: The baseline level of LDH released by intact control HuNSPCs (SC27 cells in DEP buffer not exposed to DEP force) is set as 0%. The maximum amount of LDH release is shown for HuNSPCs (SC27) lysed with triton-X100 and is set as 100% LDH released. Right panel: Release of LDH by cells after 60 sec exposure to DEP frequencies is only above background levels at 50 kHz. (E) The cellular metabolic activity of HuNSPCs (SC27) after 60 sec exposure to DEP frequencies remains greater than 90% for all conditions. Data are represented as mean ± SE (*, p-value < 0.05 and **, p-value <0.01).