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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Integr Biol (Camb). 2012 Oct;4(10):10.1039/c2ib20171b. doi: 10.1039/c2ib20171b

Fig. 5. DEP exposure does not interfere with differentiation of NSPCs into neuronal and astrocytic lineages.

Fig. 5

(A) Schematic portrays the experimental design to test effects of DEP on neuronal and astrocytic lineage potential of NSPCs. (B) Left panel: The neuronal lineage potential of SC27 HuNSPCs (as shown by generation of MAP2+ cells) is not altered by long-term DEP exposure at various DEP frequencies. Control cells were not exposed to DEP force. Right panel: Image shows HuNSPCs (SC27) differentiated for 14 days and stained with MAP2 antibody (red) to detect neurons. All nuclei are stained blue. (C) Left panel: The astrocytic lineage potential of SC27 HuNSPCs (as shown by generation of GFAP+/Sox2− cells) is not modified by long-term DEP exposure at various DEP frequencies. Right panel: Image shows HuNSPCs (SC27) differentiated for 7 days and co-stained with GFAP (red) and Sox2 (green) antibodies (GFAP+/Sox2− astrocytes are shown by arrows). All nuclei are stained blue. Data are represented as mean ± SE.