Table 1. Quiescent stem cell gene signature*.
| Function | Downregulated genes‡ | Upregulated genes‡ |
|---|---|---|
| Cell cycle progression and checkpoint control | ANLN, BIRC5, CCNA2, CCNB1, CCNE2, SGOL1 | CCND3, PDK1 |
|
| ||
| DNA replication and chromosome segregation | MCM4, PCNA, RRM2, TOP2A | |
|
| ||
| Mitochondrial function | CYCS, MTCH2, SLC25A5 | |
|
| ||
| Chromatin and nucleosome assembly | H2AFZ, HAT1 | SMARCA2 |
|
| ||
| Regulation of transcription | FOXO3, EZH1, PRDM5, PTOV1, ZFP30, ZBTB20, PHF1, CTDSP1, THRA, TEF | |
|
| ||
| RNA processing | DDX39 | DICER1 |
|
| ||
| Other | 2810417H13Rik, CAPZA1, HADHB, IDH3A, KPNA2, PGK1 | A930001N09Rik, BCAS3, DDX3Y, GABARAPL1, GLTSCR2, ITM2A, IL18, ZYX, EPHX1, CLSTN1, GSTK1, 5730403B10Rik, DDT, IVD, FHL1, NDRG2, GRINA, PIK3R1, FYN, CHKB, PINK1, ULK2, DNAJB9, PFDN5, CTSF, CRIM1, SEPP1, GABBR1, GRB10, BBS2, RPS14, IGF2R, SELENBP1, RNF167, MAP1LC3A |
*
Comparison of microarray data sets revealed a gene signature that is common to quiescent haematopoietic stem cells (HSCs), muscle stem cells (MuSCs) and hair follicle stem cells (HFSCs). Selected genes (30 out of 71 genes) are shown and grouped on the basis of pathways in which they are presumed to function. Genes exhibiting expression level changes that are shared among the stem cell compartments are listed under ‘other’. Consistent with the dormant phenotype of a quiescent stem cell, genes that are involved in cell cycle progression, DNA replication or mitochondrial functions are mostly downregulated in quiescent stem cells.
‡
For a full list of gene definitions see Supplementary information S1 (table)).