Table 1. Human primer sequences used for QRT-PCR.
| Gene | Accession No. | Primer(5′→3′) | Annealing temperature(°C ) | Product length (bp) |
| Oct-4 | NM_001173531 | (F)GCAAAGCAGAAACCCTCGTG (R)GAACCACACTCGGACCACAT |
58.8 | 173 |
| NANOG | NM_024865 | (F)AAGGTCCCGGTCAAGAAACAG (R)CTTCTGCGTCACACCATTGC |
55.7 | 237 |
| PAX6 | NM_001604 | (F)AACGATAACATACCAAGCGTGT (R)GGTCTGCCCGTTCAACATC |
62.0 | 120 |
| CK12 | NM_000223 | (F)TTCCATGTTTGGTTCTAGTTCCG (R)TCATTGCCCGAGAGAATACCT |
60.0 | 171 |
| GADPH | NM_002046 | (F)AAGGTGAAGGTCGGAGTCAAC (R)GGGGTCATTGATGGCAACAATA |
56.0 | 102 |