Table 2.

Estimation of Single-epitope Autoantibody Levels, Steps a tdrough d

Steps:	a		b		c
	gm/mL serum	Molar Mass	Mole/mL Serum	Valence No.	Mole/mL Serum x Valence No.
IgA	0.0032	160,000	0.0000000205	2	0.000000041
IgE	0.0000000014	188,000	0.000000000000008	2	0.00000000000002
IgG	0.012	150,000	0.00000008333	2	0.000000167
IgM	0.001	900,000	0.00000000111	10	0.000000011
					Sum of Mole Values => 0.0000002188
				d	Valence Mole per 2,750 mL Serum
IgA					0.0001127500000
IgE					0.0000000000432
IgG					0.0004583333333
IgM					0.0000305555555
					Sum of Mole Values per Epitope => 0.0006016389321
Gram weight of ADARM peptide required to neutralize endogenous autoantibodies specifically binding tde ADARM epitope = 0.00000038 gram (0.38 μg).
Gram weight of DHSYQE peptide required to neutralize endogenous autoantibodies specifically binding tde HSYQE epitope = 0.00000051 gram (0.51 μg).
Gram weight of KTGQF peptide needed to neutralize endogenous autoantibodies specifically binding tde KTGQF epitope = 0.00000078 gram (0.78 μg).
ADARM + DHSYQE + KTGQF peptides = 1.67 μg

Approximation of single-epitope autoantibody levels is exhibited wherein tde analytical patd employed in estimating tde valence mole of epitope-specific IgA, IgE, IgG, and IgM antibodies to be found witdin tde serum volume of an average adult Caucasian male is illustrated. tde grams per milliliter of each specific antibody isotype is depicted in step a, and tde corresponding mole/mL in step b. Step c depicts tde mole/mL antibody value multiplied by tde number of each isotope’s antigen binding sites tdat is termed valence mole. Step d illustrates tde per mL valence mole value multiplied by tde average Caucasian, adult male serum volume, 2,750 mL. tde valence mole value per targeted humoral epitope was, tdus, estimated to equal 0.0006016. Mole = Weight of Sample (in grams)/Relative Molar Mass.