Table 1.
Genes Encoding Adenylate-Forming Enzymes
| Entry | Gene(s) | Accession Number | Annotated Function | Essential (E) or Virulence Factor (V) |
|---|---|---|---|---|
| 1 | alaS, argG, argS, aspS, cysS1, gltS, glyS, hisS, ileS, leuS, lysS, lysX, metS, pheS, pheT, proS, serS, thrS, trpS, tyrS, valS | Rv2555c, Rv1658, Rv1292, Rv2572c, Rv3580c, Rv2992, Rv2357c, Rv2580c, Rv1536, Rv0041, Rv3598c, Rv1640c, Rv1007c, Rv1649, Rv1650, Rv2845c, Rv3834c, Rv2614c, Rv3336c, Rv1689, Rv2448c | Aminoacyl tRNA synthetase (Protein biosynthesis) | yes (E) |
| 2 | acs | Rv3667 | Acetyl coenzyme synthetase (glycolysis) | no |
| 3 | menE | Rv0542c | O-Succinylbenzoic acid CoA synthetase (menaquinone biosynthesis) | yes (E) |
| 4 | fadD1, fadD2, fadD3, fadD4, fadD5, fadD6, fadD7, fadD8, fadD9, fadD11, fadD12, fadD13, fadD14, fadD15, fadD16, fadD17, fadD18, fadD19, fadD35, fadD36 | Rv1750c, Rv0270, Rv3561, Rv0214, Rv0166, Rv1206, Rv0119, Rv0551c, Rv2590, Rv1550, Rv1427c, Rv3089, Rv1058, Rv2187, Rv0852, Rv3506, Rv3513c, Rv3515c, Rv2505c, Rv1193 | Fatty acyl CoA synthetase (fatty acid catabolism, cholesterol catabolism) | no (except fadD36) |
| 5 | fadD10 | Rv0099 | Possibly involved in synthesis of the nonribosomal peptide encoded by nrp (adjacent to nrp) | no |
| 5 | fadD21 | Rv1185c | Possibly involved in mycolipanoic, mycolipenic, mycolipodienic acid synthesis (adjacent to pks3/4) | no |
| 6 | fadD22 | Rv2948c | Phenolglycolipid synthesis (loading of 4-hydroxybenzoic acid onto Pks15/1) | no |
| 7 | fadD23 | Rv3826 | Phthioceronic and hydroxyphthioceronic acid portion of the sulfolipids (adjacent to pks2) | no |
| 8 | fadD24 | Rv1529 | Possibly involved in lipooligosaccharides synthesis (adjacent to pks5) | no |
| 9 | fadD26 | Rv2930 | Synthesis of the phthiocerol portion of the dimycocerosate esters (loading of fatty acid onto PpsA) | no |
| fadD28 | Rv2941 | Synthesis of the mycocerosic acid portion of the dimycocerosate esters (loading of fatty acid onto Mas) | no | |
| 10 | fadD30 | Rv0404 | Synthesis of an unknown polar lipid (loading of fatty acid onto Pks6) | yes (E) |
| 11 | fadD32 | Rv3801c | Mycolic acid synthesis (loading Pks13) | yes (E) |
| 12 | mbtM (fadD33) | Rv1345 | Mycobactin synthesis (loading of MbtL) | no (mutant slow growing) |
| 13 | fadD25, fadD29, fadD31, fadD34 | Rv1521, Rv2950c, Rv1925, Rv0035 | Unknown (fatty acyl-AMP ligases, FAALs) | no |
| 14 | nrp | Rv0101 | Multifunctional NRPS (unknown function) | no |
| 15 | mbtA, mbtB, mbtE, mbtF | Rv2384, Rv2383c, Rv2380c, Rv2379c | NRPS Adenylation domains (mycobactin biosynthesis) | yes (V) |
| 16 | nadE | Rv2438c | Glutamine dependent NAD synthetase | yes (E) |
| 17 | guaA | Rv3396c | GMP synthetase | yes (E) |
| 18 | panC | Rv3602c | Pantothenate synthetase (Coenzyme A synthesis) | yes (V) |
| 19 | mshC | Rv2130c | Cysteine:1-D-myoinosityl 2-amino-2-deoxy-D-glucopyranose ligase (mycothiol biosynthesis) | yes (E) |
| 20 | birA | Rv3279c | Biotin ligase (biotinylation of AccA1, AccD4, Pcc) | yes (E) |