Fig. 4.

Dose response of c-Jun and protein expression and phosphorylation in ST88-14 cells treated with SP600125. a ST88-14 cells were treated with SP600125 at the concentrations indicated for 16 h. Samples were analyzed by western blot and probed for the indicated protein. After each probe the blot was stripped and reprobed for the subsequent protein. Data shown are representative of three independent experiments. b The bar graph quantitatively illustrates the means ± SEM for the three experiments described in a. For phosphoc-Jun a significant inhibition (* P value < 0.05) was observed with increased concentration of SP600125 through 10 µM. 10 µM was not different from 30 µM although there was a downward trend. For total c-Jun, significant inhibition (* P value < 0.05) was observed with all increases in concentration