Skip to main content
. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Methods Mol Biol. 2012;808:10.1007/978-1-61779-373-8_21. doi: 10.1007/978-1-61779-373-8_21

Fig. 5.

Fig. 5

Overall approach to using microarrays to study antibacterial resistance that is conferred by aminoglycoside modification. The image illustrates results of binding of array-immobilized substrates that have been modified by APH(3′)-IIIa to a mimic of the bacterial rRNA A-site. The plot below the array image illustrates the decrease in affinity of the arrayed aminoglycoside upon modification by APH(3′)-IIIa; the orange bar shows the decreased signal due to the modification of kanamaycin A by APH(3′)-IIIa.