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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Carcinog. 2012 Oct 12;53(3):230–242. doi: 10.1002/mc.21969

Figure 2. Activation of VEGFR-3 in SK-N-AS neuroblastoma cells by VEGF-C156S.

Figure 2

A. SK-N-AS cells were treated with VEGF-C156S (1 nM), a specific ligand for VEGFR-3, for increasing time periods. Phosphorylation of VEGFR-3 was detected by immunoblotting. There was an increase in phosphorylation of VEGFR-3 seen after 15 minutes. B. Densitometry was done on immunoblots comparing the bands for phosphorylated VEGFR-3 to total VEGFR-3, after both were normalized to β-actin bands. These data further illustrated the findings of ligand-induced phosphorylation.