Table 2.
Methods for Profiling Small Numbers of Cells
| Method | Advantages | Disadvantages | References |
|---|---|---|---|
| Single-cell qPCR | Single cell required | Variance between cells, noise |
Taniguchi 2009 Wu 2010 |
| Single-cell RNAseq | Single cell required Transcriptome analysis | Variance between cells, noise | Tang 2009 |
| Gene-Specific PreAmp | Small sample required, pre-validated & linear amplification | Analysis of only 100 transcripts | Noutsias 2008 |
| Eberwine T7 RNA Amplification | Transcriptome analysis | Potential bias |
Van Gelder 1990 Duftner 2008 Boelens 2007 |
| RNAseq | Transcriptome analysis | Larger (>100pg) sample required |
Chen 2011 Ozsolak 2010 Tariq 2011 |