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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Curr Cancer Drug Targets. 2010 May;10(3):319–331. doi: 10.2174/156800910791190229

Fig. (4). MDM2 inhibitors augment antitumor effect of gemcitabine.

Fig. (4)

Panels A – D (Upper Panels); Capan-2 cells were exposed to either vehicle (DMSO), MI-219 (5 μM), MI-319 (5 μM), Gemcitabine (100 nM) alone, MI-219 (5 μM) + Gemcitabine (100 nM) and MI-319 (5 μM) + Gemcitabine (100 nM). Cell growth inhibition was detected using MTT assay while apoptosis was detected using Histone DNA/ELISA assay as described under Material and Methods. Error Bars denote SEM of four independent experiments. ** p value < 0.01 when compared to untreated controls. Panels A – D (Lower Panels); Isobologram analysis of the combination of gemcitabine and MI-219 or gemcitabine and MI-319 in Capan-2 cells. Combination index (CI) values were calculated using Calcusyn software. Points below the line indicate synergy.