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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Biosens Bioelectron. 2013 Jan 2;44:10.1016/j.bios.2012.12.046. doi: 10.1016/j.bios.2012.12.046

Fig. 2.

Fig. 2

E-field and Triton X-100 lysis compared for releasing exosomal GAPDH mRNA from human saliva. (a) Schematic illustration of an exosome disrupted with E-field and RNA released. (b) TEM images before (left) and after (right) E-field (top) or Triton X-100 (bottom) treatment. (i and iii) Exosomes (arrows) attached to anti-hCD63 magnetic beads; (ii and iv) Exosomes were disrupted after treating with (ii) a csw E-field for 200 s, or (iv) with Triton-X 100 for 20 min. The background is the lacey support film for TEM. (c) GAPDH mRNA by EFIRM (top) or Triton X-100 (bottom).