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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Biosens Bioelectron. 2013 Jan 2;44:10.1016/j.bios.2012.12.046. doi: 10.1016/j.bios.2012.12.046

Fig. 3.

Fig. 3

EFIRM compared to Western blot and q-PCR measurements for detection of hCD63-GFP protein and GAPDH mRNA in exosomes shed from H460 cells. (a) Titration of the GFP moiety of hCD63-GFP by EFIRM and Western blotting. (b) Titration of GAPDH mRNA by EFIRM and q-PCR. Data in (a) and (b) are fitted by a linear model. The PCR readout is presented on a log scale. The human specificity of EFIRM measurements was tested by detecting human exosomal (c) CD63-GFP protein and (d) GAPDH mRNA in mixtures of endosomes from human lung cancer H460 cells and interfering exosomes from mouse Lewis lung carcinoma LL2/LLC1 cells.